As a pollen feeder, Propylea japonica would be directly exposed to Cry proteins in Bacillus thuringiensis(Bt)-transgenic rice fields. The effect of Cry1C- or Cry2A-containing transgenic rice pollen on the fitness ofP. japonica was assessed using two dietary-exposure experiments in the laboratory. In the first experiment,larval developmental time of P. japonica was significantly longer when fed pollen from Bt rice lines ratherthan control pollen but other life table parameters were not significantly affected. In the second experiment,P. japonica was not affected when fed a rapeseed pollen-based diet containing purified Cry1C or Cry2A atconcentrations that were .10-times higher than in pollen, but P. japonica was affected when the dietcontained E-64 as a positive control. In both experiments, the stability and bioactivity of the Cry proteins inthe food sources and the uptake of the proteins by P. japonica were confirmed. The results show that P.japonica is not sensitive to Cry1C or Cry2A proteins; the effect observed in the first experiment was likelyattributable to unknown differences in the nutritional composition of Bt rice pollen. Overall, the dataindicate that the growing of Cry1C- or Cry2A-transgenic rice should pose a negligible risk to P. japonica.
简单的总结下,喂食BT水稻花粉的幼虫发育时间变长,但其他生理指标没有什么明显影响,喂食混有纯化的BT蛋白食物,则与对照组没有明显区别,说明前者的影响并非BT蛋白直接作用,可能的原因是因为BT水稻花粉的营养组成改变,但是在自然条件下,水稻花粉不是唯一食物来源,所以BT水稻花粉对龟纹瓢虫的影响忽略不计
原文链接:http://www.nature.com/srep/2015/150108/srep07679/pdf/srep07679.pdf
简单的总结下,喂食BT水稻花粉的幼虫发育时间变长,但其他生理指标没有什么明显影响,喂食混有纯化的BT蛋白食物,则与对照组没有明显区别,说明前者的影响并非BT蛋白直接作用,可能的原因是因为BT水稻花粉的营养组成改变,但是在自然条件下,水稻花粉不是唯一食物来源,所以BT水稻花粉对龟纹瓢虫的影响忽略不计
原文链接:http://www.nature.com/srep/2015/150108/srep07679/pdf/srep07679.pdf